Knowledge Management on the Novel LAGE-Like GlcNAc-Transferase Protein Family

To study a novel protein family requires a biologist to understand many different types of information. Moreover, the speed of discovery in biology has been expanding exponentially. And the public knowledge management platforms are not customized to a research project. It is often cumbersome. To overcome these obstacles, we have set up a knowledge management platform by integrating several databases. Particularly, we have constructed a knowledge management platform on the novel LARGE-like glycosyltransferase family. The predicted protein structure of a LARGE protein family member contains an N-terminal cytoplasmic domain, a transmembrane region, a coiled-coil motif and two putative catalytic domains with the conserved DXD motif and a conserved protein structural domain, as previously described. Initially, we have developed an workflow to identify the members of the LARGE-like protein family. We then integrated the DNA sequence, protein sequence, protein domain, protein family based on the CAZY databank, human and animal disease information and references into one knowledge management platform for studying diseases.